Posted: November 16th, 2015

Science

Optimisation of macromolecular crowded culture for enhanced extracellular matrix deposition

write a scientific text according to the following
Results with short description:
Brightfield images: Cell morphology
Cell viability: LDH assay (% of live cells) the data was processed,
Cell metabolic activity: alamarBlue the data was processed
Collagen type I deposition: SDS-PAGE
Collagen type V deposition: SDS-PAGE (compare the amount of collagen deposited in the presence of different concentrations of crowding, best concentration?)
Extracellular matrix deposition: collagen type I, collagen type V, fibronectin:
Immunocytochemistry (composition of the matrix and how it is affected by the crowding)

Discussion:
Discuss the results and compare between groups over time and say what is happening to the cells and also write a comparison about the Fibronectin of cells.
Brightfield images: Cell morphology (if the macromolecular crowding affects cell morphology)
Cell viability: LDH assay (% of live cells) the data was processed, I send you the graph.
Cell metabolic activity: alamarBlue (cell function, metabolic activity: replication, secreting molecules..) the data was processed and then I send you the graph.
Collagen type I deposition: SDS-PAGE (compare the amount of collagen deposited in the presence of different concentrations of crowding, best concentration?) We have to mention that the most important thing we want to see is the deposition of Collagen type I and mention the reasons.
Collagen type V deposition: SDS-PAGE (compare the amount of collagen deposited in the presence of different concentrations of crowding, best concentration?)
Extracellular matrix deposition: collagen type I, collagen type V, fibronectin: Immunocytochemistry (composition of the matrix and how it is affected by the crowding)

PLACE THIS ORDER OR A SIMILAR ORDER WITH US TODAY AND GET A GOOD DISCOUNT 🙂