Posted: April 27th, 2016

Nitrogen fixation by free living Bacteria

1. Nitrogen fixation
1.2 The nitrogenase enzyme
1.2.1. The Molybdenum Containing nitrogenase
1.2.2. The vanadium nitrogenase
1.2.3 The Iron-only nitrogenase
1.2.4 Oxygen insensitive Streptomyces thermoautotrophicus nitrogenase
1.3. Diversity of Nitrogen fixing bacteria
1.3.1 Life style of nitrogen fixers.
1.3.1.1 Symbiotic association
1.3.1.2 Associative Nitrogen fixing bacteria.
1.3.1.3. Free living nitrogen fixers.
1.3.2. Origins and evolution of nitrogenase
1.4. Nitrogen in Grass land.
1.5. Method for Evaluating of nitrogen fixation.
1.5.1. culture dependent methods.
1.5.2. culture independent methods.
1.5.2.1 the 16S r RNA gene.
1.5.2.2. the nigh gene to study the diversity of diazotrohs.
1.5.2.3 Denaturing Gradient Gel Electrophoresis (DGGE)
1.5.2.4. The acetylene reduction assay
1.5.3. the Acetylene reduction assay
1.5.4 Detecting nitrogen incorporation using stable isotope 15N2.