Posted: October 19th, 2016

Biocatalytic applications of peroxidase enzymes. Project: Chemical modification of horseradish peroxidase A2 isozyme.

Literature Survey: Biocatalytic applications of peroxidase enzymes. Project: Chemical modification of horseradish peroxidase A2 isozyme. Lit survey descriptor: Enzymes are finding increasing use as practical biocatalysts of key steps in the synthesis of small­molecule pharmaceuticals, and in other processing applications. Numerous peroxidases from a variety of sources have been used in these situations.Your literature survey should describe examples of peroxidase biocatalysts, whether naturallysourced or recombinantly­produced. Directed evolution studies, or other strategies for �improving� performance, are especially relevant. You should pay particular attention to practical applications, especially in pharma­ and/or industrially relevant examples, and to material published since 2005 (including patent specifications). Project descriptor: Plant (Class III) peroxidases have numerous applications throughout biotechnology but their thermal and oxidative stabilities often limit their usefulness. Horseradish peroxidase isoenzyme C (HRPC) has good catalytic properties and is moderately resistant to heat and to oxidation. In contrast, HRP isoenzyme A2 (HRPA2) has better oxidative but poorer thermal stability, while soybean peroxidase (SBP) displays high thermal stability. Enhancement of HRPA2�s resistance to heat (by chemical modification and/or immobilization), combined with its natural oxidative stability, could provide a robust and useful biocatalyst. You will compare the catalytic and stability properties of commercial preparations of HRPA2 and SBP. You will undertake chemical modification of HRPA2 using carbodiimide reagents that are specific for carboxyl groups (Asp, Glu residues and the C­terminus). You will also modify HRPA2 amino groups (Lys residues and the N­terminus) with anhydrides and other reagents. Your objectives are to optimize (i) the yield of HRPA2 activity post­modification, (ii) its thermal and oxidative stabilities and (iii) its catalytic performance. The host lab is experienced with numerous enzymes, especially horseradish peroxidase C (HRPC) and soybean peroxidase (SBP). It has described stabilized forms of HRPC, achieved by (i) chemical modification and (ii) recombinant means, in numerous papers. You will gain a wide and good experience of working with enzymes, chemical modification of proteins and protein stability testing. Above is the description of both literature and project research And I need you to write the literature only. In the literature: Summary (Abstract) 150 words no more Introduction Conclusion References (Harvard)